Experimental ex-vivo lung perfusion with sevoflurane: Effect on damaged donor lung grafts

OBJECTIVES: Volatile anaesthetics can provide significant protection against reperfusion injury in various experimental settings. The aim of this study was to assess the potential of sevoflurane treatment, the most commonly used volatile anaesthetic in modern anaesthesia, in rat lungs donated after circulatory death and reconditioned in an ex vivo lung perfusion (EVLP) system.METHODS: Fifteen rats were sacrificed and divided into 3 groups. In the control and sevoflurane groups, the heart-lung blocks were exposed to 1 h of warm ischaemia and 2 h of cold ischaemia and were mounted on an EVLP circuit for 3 h, in the absence or in the presence of 2% sevoflurane. In the baseline group, heart-lung blocks were harvested immediately after euthanasia. Physiological data, lung nitro-oxidative stress, lactate dehydrogenase (LDH), expression of cytokines, oedema and histopathological findings were assessed during or post-EVLP.RESULTS: The sevoflurane group showed significantly reduced LDH (8.82 +/- 3.58 arbitrary unit vs 3.80 +/- 3.02 arbitrary unit, P = 0.03), protein carbonyl (1.17 +/- 0.44 nmol.mg(-1) vs 0.55 +/- 0.11 nmol.mg(-1) , P = 0.006), 3-nitrotyrosine (197.44 +/- 18.47 pg.mg(-1) vs 151.05 +/- 23.54 pg.mg(-1) , P = 0.004), cytokine-induced neutrophil chemoattractant factor 1 (1.17 +/- 0.32 ng.mg(-1) vs 0.66 +/- 0.28 ng.mg(-1) , P = 0.03) and tumour necrosis factor alpha (1.50 +/- 0.59 vs 0.59 +/- 0.38 ng.mg(-1) , P = 0.02) when compared with the control group. In addition, sevoflurane lungs gained significantly less weight (0.72 +/- 0.09 g vs 0.72 +/- 0.09 g, P = 0.044), had less perivascular oedema (0.58 +/- 0.09 vs 0.47 +/- 0.07, P = 0.036), and improved static pulmonary compliance (+0.215 ml.cm Eta O-2(-1) , P = 0.003) and peak airways pressure (-1.33 cm Eta O-2, P = 0.04) but similar oxygenation capacity (+1.61 mmHg, P = 0.77) and pulmonary vascular resistances (+0.078 mm Eta g.min.m Iota(-1) , P = 0.15) when compared with the control group.CONCLUSIONS: These findings suggest that the potential of sevoflurane in protecting the lungs donated after cardiac death and reconditioned using EVLP could improve the outcome of these lungs following subsequent transplantation.