Uliginosibacterium Sediminicola Sp Nov., Isolated From Freshwater Sediment

Strain M1-21(T) is a Gram-stain-negative, strictly aerobic and short-rod-shaped bacterium, motile by means of a single polar flagellum; it was isolated from freshwater sediment in Korea. It grew at 10-40 degrees C (optimum 25 degrees C), pH 6.0-8.0 (optimum pH 7.0) and with 0-0.75 % (w/v) NaCl (optimal growth occurred in the absence of NaCl) on R2A agar, and it accumulated poly-beta-hydroxybutyrate granules inside the cells. According to 16S rRNA gene sequence analysis, strain M1-21(T) showed highest sequence similarity with Uliginosibacterium gangwonense (94.7 %) and Uliginosibacterium paludis (94.4 %). Phylogenetic analysis of the 16S rRNA gene sequences revealed that strain M1-21(T) belongs to the genus Uliginosibacterium. The DNA G+C content of strain M1-21(T) was 61.9 mol%. The predominant respiratory quinone was ubiquinone-8. The major fatty acids (>10 % of the total) were C-16:0 and summed feature 3 (C-16:1 omega 6c and/or C-16:1 omega 7c), and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Strain M1-21(T) showed distinct phenotypic characteristics that differentiated it from species of the genus Uliginosibacterium. Based on these results, strain M1-21(T) represents a novel species of the genus Uliginosibacterium, for which the name Uliginosibacterium sediminicola sp. nov. is proposed. The type strain is M1-21(T) (=KACC 19271(T)=JCM 32000(T)).