Anchorless Cell Surface Proteins Function As Laminin-Binding Adhesins in Lactobacillus Rhamnosus Fsmm22
FEMS microbiology letters(2017)
摘要
Anchorless cell surface proteins (CSPs) were extracted with 1 M lithium chloride solution from Lactobacillus rhamnosus FSMM22. Loss of the anchorless CSPs resulted in a 2-fold decrease in FSMM22 cells bound to a constitutive extracellular matrix glycoprotein, laminin, in vitro. DNA-binding protein HU, glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase and 30S ribosomal protein S19 (RpsS) were identified by mass spectrometry in the extract as laminin-binding adhesins. Among the four proteins, RpsS was immunohistochemically confirmed to exist on the cell surface. Our findings strongly suggest that anchorless CSPs can enhance bacterial adhesion to the host.
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关键词
cell surface proteins,host-microbial interactions,lactic acid bacteria,laminin,probiotics,ribosomal proteins
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