Immunoinformatics approaches to design a novel multi-epitope subunit vaccine against HIV infection.

•We applied novel immunoinformatics approaches to design a multi-epitope subunit vaccine.•The designed vaccine consisting of B-cell and T-cell epitopes which can induce humoral and cell mediated immune response.•Designed vaccine was found to be non-allergen, safe and potentially immunogenic in nature.•Designed vaccine has shown good interaction with TLR-3 (immune receptor) which initiates immune response in immune cell.•In silico cloning was performed to ensure vaccine expression in microbial system.