Global Characterization of Protein Complexes by Biochemical Purification-Mass Spectrometry (BP/MS).
Methods in molecular biology (Clifton, N.J.)（2018）
摘要
A proteomic platform for global analysis of protein complexes and protein-protein interactions (PPIs) is described. Briefly, after comprehensive physiochemical separation of soluble protein extracts using non-denaturing ion exchange chromatography (IEX), each fraction is subjected to quantitative tandem mass spectrometry analysis.
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关键词
High Performance Liquid Chromatography (HPLC),Intact Protein Separation,Ion Exchange Chromatography (IEX),Nano-Liquid Chromatography Tandem Mass Spectrometry (nLC-MS/MS),Protein-Protein Interaction
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