[Identification of yeasts from commercial attiéké in Abidjan (Côte d'Ivoire): Preliminary study].

C G Kouadio-Yapo, G S P Dou, N A D Aka, K D Zika,K D Adoubryn, M Dosso

JOURNAL DE MYCOLOGIE MEDICALE(2018)

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摘要
Objective. - This study aims to identify yeasts from commercial attieke. Patients and methods. - This descriptive cross-sectional survey involving 103 samples of attieke sold on the market from August to September 2017. It was conducted from the mycology laboratory of the Institut Pasteur of Cote d'Ivoire. The samples were seeded on YGC medium. The colonies of positive culture were counted and seeded again on chromogen medium. Identification of yeasts species was carried out by MALDI-TOF mass spectrometry (Vitek MS bioMerieux). Results. - As a whole, 63 samples were contaminated with fungi i.e. an overall prevalence of 61.1 %. Fifty-nine of yeasts were identified i.e. 57.2 %. Candida rugosa was most frequently isolated (62.7 %), followed by Ccandida albicans (8.5 %), Candida krusei (8.5 %) et Candida tropicalis (8.5 %). Geotrichum candidum represented 6.8 % isolated yeasts. The yeast average load is 10,124 x 10(2) CFU/g with high contamination of commercial attieke in the commune of Abobo. Conclusion. - The contamination of attieke by yeasts is a reality. C. rugosa was most frequently isolated. It would be necessary to investigate the food chain to determine the source of contamination. (C) 2018 Elsevier Masson SAS. All rights reserved.
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关键词
Contamination,Cassava,Attieke,Yeasts,Identification,Mass spectrometry,Abidjan
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