A simple micro-batch ion-exchange resin extraction method coupled with reverse-phase HPLC (MBRE-HPLC) to quantify lactoferrin in raw and heat-treated bovine milk

Sylvie Pochet,Céline Arnould, Perrine Debournoux,Jocelyne Flament,Odile Rolet-Répécaud,Eric Beuvier

Food Chemistry（2018）

引用 21|浏览21

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摘要

•Original method using resin extraction and HPLC to quantify lactoferrin in milk.•Unambiguous improvement on previous HPLC or capillary electrophoresis methods.•Analytical performances as good as or better than ELISA or RID methods.•Good correlation with immuno-based values for raw individual cow’s milk.

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关键词

AS,CV,DR,DS,ELISA,HPLC,LOD,LOQ,MBRE,pI,Phe,R1 to R4,RID,UV,α-La,β-Lg,BSA,Ig,RT

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