Genetic diversity of the human adenovirus species C DNA polymerase.

•Nucleotide and amino acids substitutions were observed respectively in 10.3% and 8.7% of HAdV C strains.•The most variable region was the NH2 terminal domain.•HAdV DNA pol sequence enabled to distinguish HAdV strains within a type.•None of the mutations associated with cidofovir or brincidofovir resistance was detected.•The mapping of HAdV DNA pol polymorphism will help for the interpretation of genotypic assays.