Effects of chelator lipids, paramagnetic metal ions and trehalose on liposomes by solid-state NMR.
Solid State Nuclear Magnetic Resonance(2018)
摘要
The effects of various lipid bound paramagnetic metal ions on liposomes prepared in the presence of trehalose and chelator lipids are evaluated to observe site-specific signal changes on liposome samples with optimal resolution in solid-state NMR spectroscopy. We found that Mn2+, Gd3+ and Dy3+ have different influences on the lipid 13C sites depending on their penetration depths into the bilayer, which can be extracted as distance information. The trehalose-liposome mixture is efficiently packed into solid-state NMR rotors and provides optimal resolution at reasonable instrument temperatures (10–50 °C). The effectiveness and convenience of the trehalose preparation for studying a membrane protein in liposomes are demonstrated by a membrane sample with a model membrane peptide to show that trehalose is useful to prepare consistent and stable membrane protein liposome samples for solid-state NMR.
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关键词
Paramagnetic relaxation effect,Solid-state NMR,Membrane proteins,Trehalose,Liposomes,Pseudo-contact shift
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