Dengue Virus IgM Serotyping by ELISA with Recombinant Mutant Envelope Proteins.

Alexandra Rockstroh,Luisa Barzon,Widuranga Kumbukgolla,Hoang Xuan Su,Erley Lizarazo,Maria Fernanda Vincenti-Gonzalez,Adriana Tami,Alice M. M. Ornelas,Renato Santana Aguiar,Daniel Cadar,Jonas Schmidt-Chanasit,Sebastian Ulbert

Emerging infectious diseases（2019）

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摘要

We developed an IgM-based ELISA that identifies the dengue virus serotype of recent infections. Dominant serotypes were detectable in 91.1% of samples from travelers and 86.5% of samples from residents of endemic regions; 97.1% corresponded to the serotype identified by PCR. This ELISA enables more accurate reporting of epidemiologic findings.

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关键词

dengue,serotypes,diagnosis,ELISA,IgM,envelope

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