High-depth fluorescence imaging using a two-photon FRET system for mitochondrial pH in live cells and tissues.
CHEMICAL COMMUNICATIONS(2018)
摘要
We developed a fluorescent pH probe (1) capable of two-photon excitation and far-visible-emission based on FRET, composed of naphthalimide-piperazine-rhodamine. It exhibited a pH-dependent reversible and fast ratiometric fluorescence change in the rhodamine emission. Probe 1 was applied to image the pH perturbations of mitochondria in living cells and tissues.
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