TLR5 silencing reduced hyperammonaemia-induced liver injury by inhibiting oxidative stress and inflammation responses via inactivating NF-κB and MAPK signals.

•TLR5 was highly expressed in liver tissues of HA rats.•The knockdown of TLR5 ameliorated HA-induced liver injury by inhibiting hepatic cell apoptosis.•TLR5 depletion inhibited HA-induced pro-inflammatory cytokine expression in liver tissues of rats.•TLR5 silencing impaired HA-induced oxidative stress responses in hepatocytes.•TLR5 downregulation inhibited HA-induced activation on TLR5/NF-κB and TLR5/MAPK signaling pathways.