Biosynthesis of Monascus pigments by resting cell submerged culture in nonionic surfactant micelle aqueous solution
Applied microbiology and biotechnology(2016)
摘要
Growing cell submerged culture is usually applied for fermentative production of intracellular orange Monascus pigments, in which accumulation of Monascus pigments is at least partially associated to cell growth. In the present work, extractive fermentation in a nonionic surfactant micelle aqueous solution was utilized as a strategy for releasing of intracellular Monascus pigments. Those mycelia with low content of intracellular Monascus pigments were utilized as biocatalyst in resting cell submerged culture. By this means, resting cell submerged culture for production of orange Monascus pigments was carried out successfully in the nonionic surfactant micelle aqueous solution, which exhibited some advantages comparing with the corresponding conventional growing cell submerged culture, such as non-sterilization operation, high cell density (24 g/l DCW) leading to high productivity (14 AU of orange Monascus pigments at 470 nm per day), and recycling of cells as biocatalyst leading to high product yield (approximately 1 AU of orange Monascus pigments at 470 nm per gram of glucose) based on energy metabolism.
更多查看译文
关键词
Resting cells,Nonionic surfactant,Extractive fermentation,Intracellular product,Monascus pigments
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络