Triterpenoids from the branch and leaf of Abies fargesii

Eighteen triterpenoids, abifarines A-R, including fourteen previously unknown and four artifacts, together with six known ones, were isolated from the branch and leaf of Abies fargesii. Their structures were elucidated by spectroscopic data analysis. The relative configurations of abifarines A and F were further confirmed by single-crystal X-ray diffraction analysis with Mo Kα irradiation. All compounds were evaluated for their in vitro cytotoxicity against the mouse cancer B16 cell line, and human cancer HepG2 and MCF7 cell lines. (24R)-cycloartane-3β,24,25-triol and (24R)-cycloartane-3β,24,25,28-tetrol showed moderate anti-proliferative effect for B16 and HepG2 cell lines with IC50 values of 32.9, 19.5 μM and 26.4, 21.5 μM, respectively.