Streptomyces Kronopolitis Sp Nov., An Actinomycete That Produces Phoslactomycins Isolated From A Millipede (Kronopolites Svenhedind Verhoeff)

A phoslactomycin-producing actinomycete, designated strain NEAU-ML8(T), was isolated from a millipede (Kronopolites svenhedind Verhoeff) and characterized using a polyphasic approach. 16S rRNA gene sequence analysis showed that strain NEAU-ML8(T) belongs to the genus Streptomyces with the highest sequence similarities to Streptomyces lydicus NBRC 13058(T) (99.39?%) and Streptomyces chattanoogensis DSM 40002(T) (99.25?%). The maximum-likelihood phylogenetic tree based on 16S rRNA gene sequences showed that the isolate formed a distinct phyletic line with NBRC 13058(T) and S. chattanoogensis DSM 40002(T). This branching pattern was also supported by the tree rconstructed with the neighbour-joining method. A combination of DNA-DNA hybridization experiments and phenotypic tests were carried out between strain NEAU-ML8(T) and its phylogenetically closely related strains, which further clarified their relatedness and demonstrated that NEAU-ML8(T) could be distinguished from NBRC 13058(T) and S. chattanoogensis DSM 40002(T). Therefore, it is concluded that strain NEAU-ML8(T) can be classified as representing a novel species of the genus Streptomyces, for which the name Streptomyces kronopolitis sp. nov. is proposed. The type strain is NEAU-ML8(T) (=DSM 101986(T)=CGMCC 4.7323(T)).