Mass cultivation of UV-B adapted Arthrospira platensis RRGK under open raceway pond for the production of Poly-β-hydroxy butyrate.

Six different strains of cyanobacteria were isolated from the freshwater lake, Arakkonam, India. Staining of cells with Nile Red showed the presence of large quantities of PHB granules in the cell cytoplasm of Arthrospira. Molecular identification of the strain was carried out using 16S rRNA analysis and their systematic position was ascertained as Arthrospira platensis RRGK. Studies were carried out on random mutagenesis approach using UV-B radiation for enhancing the production of PHB. Further, Response Surface Methodology was used for optimization of pH, temperature, and sodium bicarbonate for higher biomass and PHB production. Under open raceway pond A. platensis RRGK produced biomass concentration of 2.2 +/- 0.13g L-1 and 131 +/- 0.36 g L-1 of PHB content. It was chemically characterized through FTIR, DSC, TGA and XRD analyses. Hence, PHB can be produced from cyanobacteria by sequestering harmful CO2. It can also be used as a substitute for synthetic polymers in tissue engineering. (C) 2016 Elsevier B.V. All rights reserved.