Comparison of a Commercial Real-Time PCR Assay, RealCycler® PJIR Kit, Progenie Molecular, to an In-House Real-Time PCR Assay for the Diagnosis of Pneumocystis Jirovecii Infections.

We compared the RealCycler® PJIR kit (Progenie Molecular), available in Europe, to an in-house real-time PCR assay for the diagnosis of Pneumocystis jirovecii infections. Excellent agreement was found (concordance rate, 97.4%; Cohen's kappa, 0.918>0.8) showing that this commercial assay represents an alternative method for the diagnosis of P. jirovecii infections.