Metformin-induced endocrine disruption and oxidative stress of Oryzias latipes on two-generational condition.

•Oryzias latipes were exposed to metformin for two generations.•F0 males had increased expression of CYP19a and ERα.•Females had decreased expression of VTG2 and ERβ1 with intersex occurrence.•Metformin increased reactive oxygen species abundance.•Metformin led to oxidative stress and two-generational endocrine disruption.