An Innovative Standard Operation Procedure for Isolating GMP-Grade CD4 + CD25 + T Cells from Non-Mobilized Leukapheresis.

This SOP describes a closed system for isolating GMP-grade CD4CD25 T cells from non-mobilized leukapheresis collections (nMLCs), independent of a clean room in a certified GMP premises, by using CliniMACS format GMP grade reagents (CD25-labeled magnetic beads with/without pre-depletion of CD8 T cells and CD19 B cells), a GMP grade-A laminar hood and CliniMACS cell processing system.