Tracking of single tRNAs for translation kinetics measurements in chloramphenicol treated bacteria.

Ivan L Volkov,A Carolin Seefeldt,Magnus Johansson

Methods（2019）

引用 13|浏览10

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摘要

•Single tRNA tracking allows kinetics studies of ongoing translation in live cells.•tRNAs bind dynamically to chloramphenicol inhibited ribosomes.•Chloramphenicol acetyl transferase efficiently abolish the chloramphenicol action.

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关键词

Protein synthesis,Translation,Single-molecule tracking,tRNA,In vivo,Chloramphenicol,Chloramphenicol resistance

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