Neuronal spiking activity highlights a gradient of epileptogenicity in human tuberous sclerosis lesions.

•First recordings of spiking neuronal activity in the tuber and perituberal tissue using new hybrid electrodes equipped with tetrodes.•Gradient of epileptogenicity running from the tuber to perituberal tissue revealed by multi-scale analyses.•Observation of interactions both within and between the tuber and perituber.