Jiangella Rhizosphaerae Sp. Nov., An Actinomycete Isolated From The Rhizosphere Soil Of Wheat (Triticum Aestivum L.)

A novel actinomycete, designated strain NEAU-YY265(T), was isolated from rhizosphere soil of wheat (Triticum aestivum L.) and characterized using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NEAU-YY265(T) belonged to the genus Jiangella and exhibited 16S rRNA gene sequence similarities of 99.1 and 99.1 % to Jiangella mangrovi 3SM4-07(T) and Jiangella alkaliphila D8-87(T). The chemotaxonomic properties of strain NEAU-YY265(T) were consistent with those of the genus Jiangella: the cell-wall peptidoglycan type was based on LL-diaminopimelic acid; the whole-cell hydrolysates contained glucose, ribose and rhamnose; MK-9(H-4) and MK-9(H-2) were the predominant menaquinones. The novel strain produced branching mycelium which fragmented into short or elongated rods. The major fatty acids (>5 %) were anteiso-C-15(:0), anteiso-C-12:0, C-17(:)1 omega 8C, iso-C-16(:0), C-17(:0) and iso-C-15:0. The DNA G+C content was 72.6 mol%. Strain NEAU-YY265(T) was phenotypically distinct from recognized Jiangella species and shown to belong to a separate genomic species based on DNA-DNA hybridization results. Thus, strain NEAU-YY265(T) is considered to represent a novel species of the genus Jiangella, for which the name Jiangella rhizosphaerae sp. nov. is proposed. The type strain is NEAU-YY265(T) (=JCM 32551(T) =CG MCC 4.7475(T)).