+764 G/A Mutation of Inhibin-alpha Gene in Korean Patients with Idiopathic Premature Ovarian Failure

Objective: To evaluate the incidence of the +769 G/A mutation of inhibin- gene in Korean patients with idiopathic premature ovarian failure. Design: DNA analysis of the mutation. Methods: One hundred patients with idiopathic premature ovarian failure (POF) were recruited. Patient with known causes of premature ovarian failure were excluded: cytogenetic abnormalities, prior chemotherapy, prior bilateral oophorectomy and autoimmune disease, etc. DNA was extracted from peripheral blood and the +769 G/A variant of Inhibin- gene (INH-) was analyzed by polymerase chain reaction (PCR) and restriction fragment length polymorphism using restriction enzyme BbvI . Results: We didn’t find any case of +769 G/A variant in Inhibin- gene in 100 Korean patients with idiopathic POF. Conclusion: This result suggests that in contrary to the recent data reported on the Asian population, the +769 G/A variant in inhibin- gene may not exist in Korean patients with idiopathic POF.