Probing vaccine antigens against bovine mastitis caused by Streptococcus uberis

•FBA and EFTs are promising candidates for a bovine mastitis vaccine against Streptococcus uberis.•FBA and EFTs confer partial protection in an intraperitoneal S. uberis infection in mice.•GAPDH, FBA and EFTs have been detected in both cytoplasm and cell wall fractions of S. uberis.