Osteoblastic Cells Response to Albumin Coatings on Zinc Containing Hydroxyapatite
Key Engineering Materials(2016)
摘要
In this work, zinc-containing hydroxyapatite (ZnHA) and hydroxyapatite (HA) discs were coated with native bovine serum albumin (BSA) using 100 μg/ml BSA solution at 37°C. Changes on BSA secondary structure from α-helix to β-turn were detected by deconvolution analyses of FTIR amide I band. Fluorescence images of MC3T3-E1 osteoblast cultured on HA and ZnHA discs revealed cells with elongated morphology, typical of 2D experiments, after 24 hours. An intense cytoskeleton organization was observed on samples coated with BSA.
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关键词
albumin coatings,zinc,cells
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