Lipid raft-dependent endocytosis negatively regulates responsiveness of J774 macrophage-like cells to LPS by down regulating the cell surface expression of LPS receptors.

•Clathrin-dependent endocytosis of TLR4 does not lead to its down-regulation.•Lipid raft-dependent endocytosis reduces the cell surface level of LPS receptors.•Disruption of lipid rafts does not block LPS-stimulated cytokine production.•Inhibitors of CD14 signaling within lipid rafts do not inhibit responses to LPS.•Lipid rafts may be sites of TLR4 and CD14 sorting for endocytosis.