Process development for expansion of human mesenchymal stromal cells in a 50 L single-use stirred tank bioreactor

T. Lawson,D. Kehoe,Aletta Schnitzler,Peter J. Rapiejko,Kara Der,Kathryn Philbrick,S. Punreddy,Susan Rigby,Robert Smith,Qiang Feng,J. Murrell,M. Rook

Biochemical Engineering Journal（2017）

引用 96|浏览7

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摘要

•Human MSCs were expanded in a 50L bioreactor for 11days.•A 43-fold expansion was attained with a yield of 1.28×1010 cells.•The culture medium αMEM/PL is a xeno-free alternative to DMEM/FBS.•Human MSCs from the bioreactor display similar properties to those grown in flasks.

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关键词

Bioreactors,Mesenchymal stem cells,Microcarriers,Scale-up,Bioprocess design,Large scale cultivation

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