Characterization of intact sialylated glycopeptides and phosphorylated glycopeptides from IMAC enriched samples by EThcD fragmentation: Toward combining phosphoproteomics and glycoproteomics

Matthew S. Glover,Qing Yu,Zhengwei Chen,Xudong Shi,K. Craig Kent,Lingjun Li

International Journal of Mass Spectrometry（2018）

引用 37|浏览13

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摘要

•A method for combined analysis of the sialome and phosphoproteome is presented.•IMAC simultaneously enriches sialylated glycopeptides and phosphopeptides.•EThcD enables characterization of intact sialylated glycopeptides.•Workflow for characterizing mannose-6-phosphate-modified peptides is described.

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关键词

Phosphopeptides,Glycopeptides,Post-translational modifications,EThcD fragmentation,Phosphoproteomics,Glycoproteomics

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