Investigation of Biocide Biodegradation in Wastewater under Laboratory Set-Up in Anaerobic, Aerobic and Aerobic with Substrate Conditions

In this study the biodegradation of five biocides such as Isoproturon (IP), Octyl-isothiazolinone (OIT), Terbutryn (TB), Cybutryne, Irgarol (IRG) and Tebuconazole (TBU) in raw wastewater have been investigated. The laboratory experiment was performed under three conditions as aerobic, anaerobic and aerobic with substrate (molasses). The biocides concentration was detected using High Pressure Liquid Chromatography coupled with Mass Spectrometer (HPLC-MS). This was conducted to quantify the biodegradation of biocides in wastewater. Under these three conditions investigated Isoproturon (IP) had no clear degradation. Octyl-isothiazolinone (OIT) is significantly biodegraded in all three conditions. Terbutryn (TB), Cybutryne, Irgarol (IRG) and Tebuconazole (TBU), investigated are slowly biodegradable. From the experiment, it is observed that aerobic with substrate condition is most suitable for biodegradation of the selected biocides.