Homology threading to generate RNA polymerase structures

Yunsoo Kim,Nils Benning,Kasey Pham, Nahid Baghdadi, Gino Caruso, Megan Colligan, Allyssa Grayson, Alexis Hurley, Nick Ignatoski,Sandra Mcclure, Kelsey Mckaig,Emily Neag, Cole Showers, Alexis Tangalos, Jessica Vanells,Kaillathe Padmanabhan,Zachary F. Burton

Protein Expression and Purification（2018）

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摘要

Homology threading is a powerful technology for generating structural models based on homologous structures. Here we use threading to generate four complex RNA polymerase models. The models appear to be as useful as x-ray crystal structures or cryo-electron microscopy structures to support research projects.

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关键词

Homology modeling,Homo sapiens RNA polymerase I and III,Drosophila melanogaster RNA polymerase II preinitiation complex

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