Automation of multi-analyte prostate cancer biomarker immunoassay panel from whole blood by minimum-instrumentation rotational flow control

•Multi-step sample-to-answer process automation using purely rotational flow control.•Dissolvable film valve release-frequency definition enhanced more than 3 fold over state-of-the-art.•Novel valve architecture for significantly higher integration density of operations.•Triplex on-disc detection of Prostate cancer multi-biomarker panel using ELISA with controls.•Flexibility of design for operation of variants of the ELISA format.