The association of low molecular heparin and galectin-3 on the cell migration and proliferation of vascular endothelial cell from mesenchymal stem cells

Objective: To explore the effect of the association of low molecular heparin and Galectin-3 on the cell migration and proliferation of vascular endothelial cell from mesenchymal stem cells.Methods: Depending on the administration, this study is divided into four groups: low molecular weight heparin group, adding 20 μg/ml low molecular weight heparin into the cells; Galectin-3 group, adding 5 μg/ml Galectin-3 into the cells; combination group, adding 20 μg/ml low molecular weight heparin and 5 μg/ml of Galectin-3 into the cells; control group, equal volume of phosphate buffer saline buffer into the cells. Then we explored the effect of the association of low molecular heparin and Galectin-3 on the cell migration and proliferation of vascular endothelial cell from mesenchymal stem cells.Results: The optical density at 490 nm (OD490) for LMWH, Galectin-3, combined and control groups were 0.285 ± 0.018, 0.297 ± 0.041, 0.351 ± 0.016, and 0.233 ± 0.005, respectively, and the combined group could significantly increase the cell proliferation than another group (Pu003c0.05). Cultured for 24 h, the cell migration rate of low molecular weight heparin group and Galectin-3 group were 42.02 ± 7.62 and 45.82 ± 3.96, respectively, whereas the cell migration rate of combined group and control group were 68.53 ± 11.22 and 34.21 ± 3.99, respectively, suggesting that combined group had the largest cell migration (Pu003c0.05).Conclusion: The association of low molecular heparin and Galectin-3 could significantly improve the cell migration and proliferation of vascular endothelial cell from mesenchymal stem cells.