MP72-15 CHARACTERIZATION OF ONCOLYTIC MEASLES VIRUS IN PATIENT-DERIVED RENAL CELL CARCINOMA XENOGRAFTS GROWN ON CHICKEN CHORIOALLANTOIC MEMBRANES AS A MODEL FOR EARLY METASTATIC DISEASE

INTRODUCTION AND OBJECTIVES: Clear cell renal cell carcinoma (ccRCC) is characterized by a metabolic feature : an energy production by aerobic glycolysis at the expense of oxydative phosphorylation [1].Ketogneic diet (KD), which consists of high fat and low carbohydrate intakes, could bring required energy substrates to healthy cells while depriving tumor cells of glucose.METHODS: We study the effect of ketogenic diet on a ccRCC cell line : ACHN.Fifteen CD-1 nude mice received a sub-cutaneous xenograft of ACHN cells, and were then split into 3 feeding groups and fed either a standard diet (SD group, n¼5), a 2:1 ketogneic diet (KD 2:1 group, n¼5) or a 4:1 ketogenic diet (KD 4:1 group, n¼5) ad libitum.Tumor growth, blood glucose and blood ketone levels were measured weekly for 8 weeks.RESULTS: Ketosis was quickly reached.The mean blood ketone level was 0,86 mmol/L in SD group, 1,07 mmol/L in KD 2:1 group and 1,21 mmol/L in KD 4:1 group (p<0,01) (Fig. 1).The mean 8-week tumor growth was 351% in SD group, 65% in KD 2:1 group and 66% in KD 4:1 group (p¼0,01) (Fig. 2).There was no difference in weight increase between the 3 groups.CONCLUSIONS: This study showed that ketogenic diet can slow ccRCC tumor growth in a mouse model, without any difference of result between 2:1 or 4:1 KD.These outcomes have to be verified in other cell lines, and signalling pathways need to be understood by transcriptomic and metabolomic analyses, before starting clinical trials.