Notoginsenoside R-1 Prevents H9c2 Cardiomyocytes Apoptosis Against Hypoxia/Reoxygenation Via The Ers/Pi3k/Akt Pathway

Notoginsenoside R-1 (NGR(1)) is separate from Panax notoginsenosides (PNS), and plays a role similar to phytoestrogen in preventing and treating cardiovascular diseases. However, the protective mechanism of NGR(1) in the myocardial ischemia/reperfusion injury via the estrogen receptor (ER) pathway remains unclear, which hinder its application. This study aimed to study the preventive mechanisms of NGR(1) in the apoptosis of H9c2 cardiomyocytes after hypoxia/reoxygenation (H/R). NGR(1) did not affect the expression of ER and ER proteins in normal H9c2 cardiomyocytes. However, NGR(1) could upregulate the ER and G protein-coupled receptor 30 (GPR30) proteins in H9c2 cardiomyocytes after H/R without affecting ER levels. Moreover, it significantly affected the expression levels of PI3K and its downstream apoptosis proteins such as Bcl-2 Associated X Protein (Bax), B cell lymphoma/lewkmia-2 (Bcl-2), caspase-3, and so forth. Whereas, after adding the PI3K protein antagonist, the modulatory expression levels of PI3K and its downstream apoptosis proteins were remarkably abolished. After adding ER and GPR30 antagonists, NGR(1) had no significant effect on the expression of PI3K and its downstream Akt protein in the model group. The data of flow cytometry showed that after adding the ER, GPR30 and PI3K antagonists, the apoptotic rate of cardiomyocytes had no significant changes compared with the model group. This study demonstrated that NGR(1) protected H9c2 cardiomyocytes from the injury after H/R by affecting ER and GPR30 to regulate the expression levels of PI3K and its downstream apoptosis proteins.