Decorating Mn 3 O 4 nanoparticle on NiO nanoflake arrays for high-performance electrochemical biosensors

Substrate materials play a significant role in the improvement of electrochemical biosensors. In the present work, NiO nanoflake arrays were fully and uniformly decorated with Mn 3 O 4 nanoparticles by stepwise method. The as-prepared samples were characterized by scanning electron microscopy (SEM), x-ray diffractionmeter (XRD), transmission electron microscopy (TEM), x-ray photoelectron spectroscopy (XPS), energy-dispersive x-ray spectroscopy (EDS), and chronoamperometry. Subsequently, the optimized Mn 3 O 4 nanoparticle/NiO nanoflake arrays were employed as substrate to fabricate electrochemical biosensors for glucose determination. The results demonstrated that as-prepared glucose biosensors could achieve 226.2 μA mM −1 cm −2 of sensitivity and 1.0 μM of detection limit, respectively. In addition, the linear range was between 9.9 and 3665.0 μM. The developed substrate could also be extended to construct electrochemical biosensors based on other oxidase with excellent performance.