Patch-Clamp Recordings from Human Embryonic Stem Cells-Derived Fragile X Neurons.
Methods in molecular biology (Clifton, N.J.)(2019)
摘要
Performing electrophysiological recordings from human neurons that have been differentiated in vitro, as compared to primary cultures, raises many challenges. However, patch-clamp recording from neurons derived from stem cells provides an abundance of valuable information, reliably and fast. Here, we describe a protocol that is used successfully in our lab for recording from both control and Fragile X neurons, derived in vitro from human embryonic stem cells.
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关键词
Current-clamp,In vitro electrophysiological recording,Patch-clamp,Voltage-clamp,Whole-cell
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