First isolation of Sarcocystis caninum sarcocysts from two domestic dogs ( Canis familiaris ) from China

The presence of Sarcocystis cysts in the muscle tissue of domestic dogs ( Canis familiaris ), which normally serve as definitive hosts, is unusual and infrequent. Here, S . caninum sarcocysts were identified for the first time in two of 37 dogs (2.7%) from China. Examination using light microscopy found that the S . caninum sarcocysts were up to 1520 μm long and up to 147 μm wide and contained numerous 1.5–3.3 μm wedge-like villar protrusions (vp). Transmission electron microscopy revealed that the sarcocysts had pleomorphic vp that closely resembled those of “type 9c.” Five loci, 18S rDNA, 28S rDNA, mitochondrial cox 1, ITS1 and ropB , were sequenced and characterized in S . caninum sarcocysts. The sequences of the five loci shared similarities of 99.9–100%, 99.0–100%, 99.4–100%, 99.6–100%, and 99.7–100%, respectively, with those of S. arctica . Phylogenetic analysis based on the sequences of 28S rDNA and mitochondrial cox 1 indicated that S . caninum and S. arctica are closely related to Sarcocystis species that use a raptorial bird as their definitive host.