Morphology and molecular analysis of Paratylenchus chongqingensis n. sp. (Nematoda: Paratylenchinae) from soil associated with Ophiopogon japonicus in China

Paratylenchus chongqingensis n. sp. extracted from the soil associated with Ophiopogon japonicus in China is described and illustrated. The new species is characterized by having the female with a body length of 243–274.5 μm, stylet 21–24.5 μm long, lip region distinctly set off forming a flattened labial disc, lateral field with three lines, lateral vulval flaps distinct, tail with a beak-like and subacute terminus; male with a 224.5–246.5 μm long body, spicules 13–14.5 μm long, stylet lacking and pharynx degenerate, tail tapering gradually, and then becoming narrow abruptly near the terminus, forming a short pointed filiform tail tip. The internal transcribed spacer sequences of the ribosomal RNA (ITS rRNA) gene of the new species were amplified and sequenced. The phylogenetic relationships of this new species with other Paratylenchus spp. using the ITS rRNA gene is given in this study.