Optimization of a Biosurfactant Production from Bacteria Isolated from Soil and Characterization of the Surfactant / Topraktan Izole Edilen Bakterilerden Biyosurfaktan Üretiminin Optimizasyonu Ve Surfaktanın Karakterizasyonu

Objective: Biosurfactants are surface-active substances produced by microorganisms. The growth of microorganism supports biosurfactant production on hydrocarbon polluted environments. In this study, the capacity of biosurfactant production of Georgenia daeguensis (accession number, KP798810) isolated from hydrocarbon-contaminated soil was studied.Methods: The components of biosurfactant production medium were investigated through Plackett-Burman methodology. By using Placket-Burman based on statistical screening, six of the eleven factors of production medium were found to be critically effective on the biosurfactant production.Results: The significant factors were KCl, ZnSO4 center dot 7H(2)O, CaCl2 center dot 2H(2)O, KH2PO4, MgSO4 center dot 7H(2)O and glucose. Among of these, KCl had the highest effect contribution with 55.22%. After the optimization by such design, critical micelle concentration value was reached to 2.2 g L-1. When anti-bacterial activity of biosurfactant was screened, it showed antibacterial effect against to Klebsiella pneumoniae. Besides, the biosurfactant was characterized by FT-IR, critic micelle concentration and zeta analysis.Conclusion: This study reported that Georgenia daeguensis is able to produce effective biosurfactant exhibiting antibacterial activity against K. pneumoniae.