Enhanced catalytic properties of novel (αbγ)2 heterohexameric Rhodobacter capsulatus xanthine dehydrogenase by separate expression of the redox domains in Escherichia coli
Biochemical Engineering Journal(2017)
摘要
•Novel method to translate active (αβγ)2 XDH by separate expression of redox domains.•Two (αβγ)2 variants of R. capsulatus XDH constructed by splitting its small subunit.•Both (αβγ)2 R. capsulatus XDH variants increased the thermostability by 11°C.•A split variant enhanced the turnover number and catalytic efficiency.•The first in vivo biosynthesis of active split (αβγ)2 XDH with improved properties.
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关键词
Genetic engineering,Proteolysis,Redox center,Rhodobacter capsulatus,Split protein,Xanthine dehydrogenase
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