Enzyme and Cancer Cell Selectivity of Nanoparticles: Inhibition of 3-D Metastatic Phenotype and Experimental Melanoma by Zinc Oxide
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY(2017)
摘要
Biomedical applications for metal and metal oxide nanoparticles are rapidly increasing. Here their functional impact on two well-characterized model enzymes, Luciferase (Luc) or beta-galactosidase (beta-Gal) was quantitatively compared. Nickel oxide nanoparticle (NiO-NP) activated beta-Gal (> 400% control) and boron carbide nanoparticle (B4C-NP) inhibited Luc (< 10% control), whereas zinc oxide (ZnO-NP) and cobalt oxide (Co3O4-NP) activated beta-Gal to a lesser extent and magnesium oxide (MgO) moderately inhibited both enzymes. Melanoma specific killing was in the order; ZnO > B4C >= Cu > MgO > Co3O4 > Fe2O3 > NiO, ZnO-NP inhibiting B16F10 and A375 cells as well as ERK enzyme (> 90%) and several other cancer-associated kinases (AKT, CREB, p70S6K). ZnO-NP or nanobelt (NB) serve as photoluminescence (PL) cell labels and inhibit 3-D multi-cellular tumor spheroid (MCTS) growth and were tested in a mouse melanoma model. These results demonstrate nanoparticle and enzyme specific biochemical activity and suggest their utility as new tools to explore the important model metastatic foci 3-D environment and their chemotherapeutic potential.
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关键词
Metal Oxide Nanoparticle (MONP),beta-Galactosidase (beta-Gal),Luciferase (Luc),Two Dimensional Fluorescence,Difference Spectroscopy (2-D FDS),Photoluminescence (PL),Multi-Cellular Tumor Spheroids (MCTS),Nano-Belt (NB)
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