Purification of Total Flavonoids of Abelmoschus Manihot (L.) Medic and Its Antioxidant Properties in Vivo

In order to optimize the purification conditions and the antioxidant properties in vivo of total flavonoids from Abelmoschus manihot (L.) Medic, AB-8 macroporous resin is used for static adorption and elution. The optimum purification conditions are as follows: Sample solution concerntration of 0.1 g/mL, pH 3.5, 70% ethanol as eluant, 20∶1(mL/g) of eluant volum to resin weight ratio. The results in the optimum purification conditions show that the purity of TFA reaches 48.50%. There are no significant difference in indexes of mice organs between experimental groups and normal control group (P＞005). MDA content exhibits a significant decrease both in serum and liver of mice (P＜0.05). While the level of CAT, GSH-Px and SOD activities in serum and liver are higher in experimental groups than those in normal control group showing dose-response relationship. These results indicate that TFA has good antioxidant properties in vivo.