Structure Characterisation Of Polysaccharide Isolated From Huangjiu And Its Anti-Inflammatory Activity Through Mapk Signalling

Huangjiu is a famous alcoholic beverage in China due to its nutritional and functional properties. In the present study, a polysaccharide HJ-P with molecular weight of 1435 Da was purified from huangjiu and was composed of rhamnose, arabinose, mannose, glucose, galactose and glucose (highest mole percentage of 85.36%). Methylation and NMR analysis suggest that the linkage type of HJ-P was proposed to be composed of (1 -> 4)-linked-Glcp, (1 -> 6)-linked-Galp and (1 -> 4)-linked-Manp with terminal unit (1 ->)-linked-Glcp and most of the sugar residues were alpha-anomeric configuration. HJ-P had a great anti-oxidant activity and the scavenging rates of DPPH and ABTS radical were 92.61% and 78.13% respectively. Meanwhile, HJ-P effectively reduced NO and pro-inflammatory cytokines production through the phosphorylation inhibition of MAPK signal pathway. This is the first report on anti-inflammatory activity of polysaccharide from huangjiu and will be helpful to gain better understanding of the functional value of huangjiu.