Bioremediation process of oil spill using fatty-lignocellulose sawdust and its enhancement effect

The present paper deals with the treatment of oil spills using weights of modified lignocellulose sawdust. The crude oil sorption on the surface of sawdust was discussed. Nineteen crude oil-degrading bacterial isolates were separated from an oil-polluted area in Saryaqos, Al-kanakah and Mustorud area, Egypt. Four bacterial species showed the predominated growth rate on crude oil hydrocarbons. The effect of the different weight of sawdust (0.1–0.5 w/v) on the bacterial degradation of the crude oil sample was investigated. Biodegradation potential was evaluated after 5 days. The total oil was removed from the microcosms after the biological treatment ranging from 65 to 80% after 5 days. Gas Chromatographic (GC) analysis of the crude oil remaining in the culture medium, showed that the higher biodegradation of isoparaffins than n-paraffins in microcosms containing biosurfactant, the higher weight of fatty sawdust (FSD) at 0.5 g and BI1, BI4 and a bacterial consortium of four bacterial isolates separately. The result shows that these bacterial strains can be used for the bioremediation using modified sawdust in oil-polluted area.