PGE2/EP4 receptor and TRPV1 channel are involved in repeated restraint stress-induced prolongation of sensitization pain evoked by subsequent PGE2 challenge

•3d restraint stress prolonged sensitization pain evoked by subsequent PGE2 challenge.•3d restraint stress increased levels of CORT, COX2, EP4 and TRPV1 in peripheral tissues.•3d CORT injection prolonged sensitization pain evoked by subsequent PGE2 challenge.•Antagonists of GR, EP4 or TRPV1 prevented 3d restraint stress prolonged sensitization pain.•CORT increased EP4 and TRPV1 protein levels in DRG cultures.