Efficient generation of adenovirus vectors carrying the Clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR associated proteins (Cas)12a system by suppressing Cas12a expression in packaging cells.

Tomohito Tsukamoto,Eiko Sakai,Fumitaka Nishimae,Fuminori Sakurai,Hiroyuki Mizuguchi

Journal of Biotechnology（2019）

引用 3|浏览4

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摘要

•Production of Ad vectors was suppressed by expression of Cas12a.•Regulation of Cas12a expression by Tet-on system or conditional promoter improved the titer of Ad vectors.•Suppression of Cas12a expression in packaging cells improved the titer of Ad vectors.

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关键词

Clustered regularly interspaced short palindromic repeat,Cas12a,Adenovirus vector

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