Il-4 Mediated Resistance Of Balb/C Mice To Visceral Leishmaniasis Is Independent Of Il-4r Alpha Signaling Via T Cells

Previous studies infecting global IL-4R alpha(-/-), IL-4(-/-), and IL-13(-/-) mice on a BALB/c background with the visceralizing parasite Leishmania donovani have shown that the T helper 2 cytokines, IL-4, and IL-13, play influential but not completely overlapping roles in controlling primary infection. Subsequently, using macrophage/neutrophil-specific IL-4R alpha deficient BALB/c mice, we demonstrated that macrophage/neutrophil unresponsiveness to IL-4 and IL-13 did not have a detrimental effect during L. donovani infection. Here we expand on these findings and show that CD4+ T cell-(Lck(cre)), as well as pan T cell-(iLck(cre)) specific IL-4R alpha deficient mice, on a BALB/c background, unlike global IL-4R alpha deficient mice, are also not adversely affected in terms of resistance to primary infection with L. donovani. Our analysis suggested only a transient and tissue specific impact on disease course due to lack of IL-4R alpha on T cells, limited to a reduced hepatic parasite burden at day 30 post-infection. Consequently, the protective role(s) demonstrated for IL-4 and IL-13 during L. donovani infection are mediated by IL-4R alpha-responsive cell(s) other than macrophages, neutrophils and T cells.