PacBio Amplicon Sequencing Method To Measure Pilin Antigenic Variation Frequencies of Neisseria gonorrhoeae

Egon A. Ozer,Lauren L. Prister,Shaohui Yin, Billy H. Ward,Stanimir Ivanov,H Steven Seifert, Vincent B. Young

MSPHERE（2019）

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摘要

Diversity generation systems are used by many unicellular organism to provide subpopulations of cell with different properties that are available when needed. We have developed a method using the PacBio DNA sequencing technology and a custom computer program to analyze the pilin antigenic variation system of the organism that is the sole cause of the sexually transmitted infection, gonorrhea.

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关键词

antigenic variation,Pacific Biosciences sequencing,amplicon sequencing,gene diversification

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