Preparation of Synthetic Amanitin Epitope Imprinted Polymers via Thiol-ene Click Reaction for Recognition and Extraction α- and β-Amanitins from Mushrooms

Extraction and purification of α- and β-amanitins from mushroom samples are still challenges. Surface-imprinted core–shell microspheres were synthesized combining target recognition determinant imprinting and thiol-ene click reaction, and applied to the extraction of α- and β-amanitins in mushroom samples. The highly efficient thiol-ene click reaction method offers a simple condition during the crosslinking process of molecularly imprinted polymers (MIP), and the functional monomer using could be successfully introduced onto the silica microspheres. The synthesized SiO 2 @MIP were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR), and thermogravimetric analysis (TGA). The measurement of SiO 2 @MIP resultants using high-performance liquid chromatography (HPLC) clearly demonstrates that SiO 2 @MIP not only display excellent affinity, but also show good selective recognition to α- and β-amanitins. The imprinted microspheres were successfully applied as sorbents for the extraction of α- and β-amanitins from mushrooms, attaining recoveries of 76.8–79.2%. Furthermore, the samples were effectively purified according to the chromatogram. Graphic Abstract