Nocardia Stercoris Sp. Nov., A Novel Actinomycete Isolated From The Cow Dung

A novel actinobacterial strain, designated NEAU-LL90(T), was isolated from cow dung collected from Shangzhi, Heilongjiang Province, north-east PR China and characterized by using a polyphasic approach. Morphological and chemotaxonomic characteristics were consistent with those members of the genus Nocardia. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The predominant menaquinone detected was MK-8(H4, omega-cycl). Major fatty acids (>10 %) were identified as C-16:0, C-18:1 omega 9c, C-18:0 and 10-methyl C-19:0. Mycolic acids were present. The results of 16S rRNA gene sequence analysis showed that strain NEAU-LL90(T) belongs to the genus Nocardia with high sequence similarity to Nocardia niigatensis JCM11894(T) (98.1 %), similarities to other type strains of species of the genus Nocardia were found to be lower than 98.0 %. Furthermore, a combination of DNA-DNA hybridization results and some phenotypic characteristics demonstrated that strain NEAU-LL90(T) could be distinguished from its closest relative. Therefore, it is proposed that strain NEAU-LL90(T) represents a novel species of the genus Nocardia, for which the name Nocardia stercoris sp. nov. is proposed. The type strain is NEAU-LL90(T) (=CGMCC 4.7500(T)=JCM 32663(T)).